Compound pollution refers to two or more kinds of pollutants with different properties, a pollutant from different sources, or the simultaneous existence of two or more different types of pollutants in the same environment. In this study, we aimed to investigate the individual and combined toxicity of the insecticide imidacloprid (IMI), the herbicide acetochlor (ACT), and the fungicide tebuconazole (TBZ) to zebrafish. The acute toxicity test results showed that the 96-h LC50 values of IMI, ACT, and TBZ were 276.84 (259.62–294.35) mg active ingredient (a.i.) L−1, 1.52 (1.34–1.74) mg a.i. L−1, and 8.16 (7.7–8.6) mg a.i. L−1, respectively. The combinations of IMI, ACT, and TBZ with toxicity ratios of 1:2:2, 1:4:4, 2:4:1, and 4:1:4 displayed synergistic toxic effects on zebrafish, while the toxicity ratios of 1:1:1, 1:1:2, 2:1:2, 2:2:1, and 4:2:1 of IMI, ACT, and TBZ, respectively, exhibited antagonistic toxic effects on zebrafish. The following experiments were performed with a toxicity ratio of 1:4:4 (IMI:ACT:TBZ). The activities of four enzyme biomarkers related to oxidative stress in the liver, catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), and malondialdehyde (MDA) content were evaluated in each exposure group on days 7, 14, 21, and 28. Compared with those of the control group, the activities of CAT, SOD, and GST and the MDA content were significantly altered at different time points in the individual and combined exposure groups. Additionally, the activities of CAT, SOD, and GST and the MDA content were significantly altered in the combined group compared with those of the individual group after 14 days or 21 days of exposure. Therefore, it was confirmed that combined toxicity studies are indispensable in risk assessment. 相似文献
Wildlife provides food, medicine, clothing, and other necessities for humans, but overexploitation can disrupt the sustainability of wildlife resources and severely threaten global biodiversity. Understanding the characteristics of consumer behavior is helpful for wildlife managers and policy makers, but the traditional survey methods are laborious and time-consuming. In contrast, culturomics may more efficiently identify the features of wildlife consumption. As a case study of the culturomics approach, we examined tiger bone wine consumption in China based on social media and Baidu search engine data. Tiger bone wine is one of the most purchased tiger products; its consumption is closely related to tiger poaching, which greatly threatens wild tiger survival. We searched a popular social media website for the term “tiger bone wine” and focused on posts that were originally created from 1 January 2012 to 31 December 2018. We filtered and classified posts related to the purchase, sale, or consumption of tiger bone wine and extracted information on providers, consumption motivations, year of production, and place of origin of the tiger bone wines based on the texts and photos of these posts. We found 756 posts related to tiger bone wine consumption, 113 of which mentioned providers of tiger bone wine, including friends (53%), elder relatives (37%), peer relatives (7%), and others (3%). Out of the 756 posts, 266 indicated the motivations of tiger bone wine consumption. Tiger bone wines were consumed as a tonic (34%), medicine (23%), game product (30%), and a symbol of wealth (28%). Some posts indicated ≥2 consumption motivations. These findings were consistent with the search queries from Baidu index. Such information could help develop targeted strategies for tiger conservation. The culturomics approach illustrated by our study is a rapid and cost-efficient way to characterize wildlife consumption. 相似文献
Phthalates (PAEs) in drinking water sources such as the Yangtze River in developing countries had aroused widespread concern. Here, the water, suspended particulate matter (SPM), and sediment samples were collected from 15 sites in wet and dry seasons in Zhenjiang, for the determination of six PAEs (DMP, DEP, DIBP, DBP, DEHP, and DOP) using the solid-phase extraction (SPE) or ultrasonic extraction coupled with gas chromatography-mass spectrometry (GC-MS). The total concentrations of six PAEs (Σ6PAEs) spanned a range of 2.65–39.31 μg L?1 in water, 1.97–34.10 μg g?1 in SPM, and 0.93–34.70 μg g?1 in sediment. The partition coefficients (Kd1) of PAEs in water and SPM phase ranged from 0.004 to 3.36 L g?1 in the wet season and from 0.12 to 2.84 L g?1 in the dry season. Kd2 of PAEs in water and sediment phase was 0.001–9.75 L g?1 in the wet season and 0.006–8.05 L g?1 in the dry season. The dominant PAEs were DIBP, DBP, and DEHP in water and SPM, DIBP, DEHP, and DOP in sediment. The concentration of DBP in water exceeded the China Surface Water Standard. The discharge of domestic sewage and industrial wastewater might be the main potential sources of PAEs. The risk quotient (RQ) method used for the risk assessment revealed that DBP (0.01 < RQ < 1) posed a medium risk, while DIBP and DEHP (RQ > 1) posed a high environmental risk in water, DIBP (RQ > 1) also showed a high risk in sediment.
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